---
title: Integration of deep learning-based histopathology and transcriptomics reveals key genes associated with fibrogenesis in patients with advanced NASH
description: Integration of deep learning-based histopathology and transcriptomics reveals key genes associated with fibrogenesis in patients with advanced NASH
---

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# [Integration of deep learning-based histopathology and transcriptomics reveals key genes associated with fibrogenesis in patients with advanced NASH](https://www.pathai.com/resources/integration-of-deep-learning-based-histopathology-and-transcriptomics-reveals-key-genes-associated-with-fibrogenesis-in-patients-with-advanced-nash)

 Written by [Admin](https://www.pathai.com/resources/author/admin) | Apr 18, 2023 4:00:00 AM

## Article

 Nonalcoholic steatohepatitis (NASH) is the most common chronic liver disease globally and a leading cause for liver transplantation in the US. Its pathogenesis remains imprecisely defined. We combined two high-resolution modalities to tissue samples from NASH clinical trials, machine learning (ML)-based quantification of histological features and transcriptomics, to identify genes that are associated with disease progression and clinical events. A histopathology-driven 5-gene expression signature predicted disease progression and clinical events in patients with NASH with F3 (pre-cirrhotic) and F4 (cirrhotic) fibrosis. Notably, the Notch signaling pathway and genes implicated in liver-related diseases were enriched in this expression signature. In a validation cohort where pharmacologic intervention improved disease histology, multiple Notch signaling components were suppressed. Jake Conway, 1,4 Maryam Pouryahya, 1,4 Yevgeniy Gindin, 2 David Z. Pan, 2 Oscar M. Carrasco-Zevallos, 1 Victoria Mountain, 1 G. Mani Subramanian, 3 Michael C. Montalto, 1 Murray Resnick, 1 Andrew H. Beck, 1 Ryan S. Huss, 3 Robert P. Myers, 3 Amaro Taylor-Weiner, 1 Ilan Wapinski, 1,5,* and Chuhan Chung 2,*

1PathAI, Boston, MA, USA   
2Gilead Sciences, Inc., Foster City, CA, USA   
3OrsoBio, Palo Alto, CA, USA   
4These authors contributed equally   
5Lead contact   
*Correspondence: ilan.wapinski@pathai.com (I.W.), chuhanchung@inipharm.com (C.C.)   
https://doi.org/10.1016/j.xcrm.2023.101016

[View Publication](https://www.cell.com/cell-reports-medicine/fulltext/S2666-3791%2823%2900126-X)

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