---
title: "Impact of Preanalytical and Analytical Factors on AI - based Tumor Cell Content Quantification for Molecular Tumor Profiling: A Real -World Study"
description: "Impact of Preanalytical and Analytical Factors on AI - based Tumor Cell Content Quantification for Molecular Tumor Profiling: A Real -World Study"
---

[PathAI Publications ](https://www.pathai.com/publications)

# [Impact of Preanalytical and Analytical Factors on AI - based Tumor Cell Content Quantification for Molecular Tumor Profiling: A Real -World Study](https://www.pathai.com/publications/impact-of-preanalytical-and-analytical-factors-on-ai-based-tumor-cell-content-quantification-for-molecular-tumor-profiling-a-real-world-study)

 Written by [Admin](https://www.pathai.com/publications/author/admin) | Nov 20, 2025 5:00:00 AM

## Summary

University Hospital Zurich(USZ) conducted a real-world evaluation of AI-based tumor cell content (TCC) quantification in routine molecular profiling, analyzing 283 digitized H&E samples across five cancer types. The study reported correlations of Rs=0.41 between pathologist and AI estimates, Rs=0.48 between pathologist and bioinformatics estimates, and Rs=0.61 between AI and bioinformatics estimates, across cancer origins and sample types. The authors conclude that high cellularity (tumor and non-tumor), technical artifacts, and necrosis contribute to discrepancies between pathologist vs AI and bioinformatics vs AI assessments and can reduce the overall performance of AI-based TCC estimation; they also emphasize that pre-analytical standardization and considerations such as specimen type and cellularity are central to implementing AI-based TCC assessment in molecular pathology workflows.

 

## Conference

 

SGPath/SSPath 2025

[View Publication](https://drive.google.com/file/d/1SLIuDIwQKXNwLQw1bAon8puGmvQyqvgb/view)

[View full post](https://www.pathai.com/publications/impact-of-preanalytical-and-analytical-factors-on-ai-based-tumor-cell-content-quantification-for-molecular-tumor-profiling-a-real-world-study)

```json
{
  "@context" : "http://schema.org",
  "@type" : "BlogPosting",
  "author" : {
    "@type" : "Person",
    "name" : "Admin"
  },
  "dateModified" : "2026-02-03T17:40:41.141Z",
  "datePublished" : "2025-11-20T05:00:00Z",
  "headline" : "Impact of Preanalytical and Analytical Factors on AI - based Tumor Cell Content Quantification for Molecular Tumor Profiling: A Real -World Study",
  "image" : {
    "@type" : "ImageObject",
    "height" : 60,
    "url" : "/hs/hsstatic/content_shared_assets/static-1.4092/img/default-amp-logo.png",
    "width" : 60
  },
  "mainEntityOfPage" : "https://www.pathai.com/publications/impact-of-preanalytical-and-analytical-factors-on-ai-based-tumor-cell-content-quantification-for-molecular-tumor-profiling-a-real-world-study",
  "publisher" : {
    "@type" : "Organization",
    "logo" : {
      "@type" : "ImageObject",
      "height" : 60,
      "url" : "/hs/hsstatic/content_shared_assets/static-1.4092/img/default-amp-logo.png",
      "width" : 60
    },
    "name" : "PathAI Publications"
  }
}
```