Targeted Solid Tumor Sequencing Panel
Table of Contents
The Targeted Solid Tumor Sequencing Panel utilizes the Illumina TruSight Tumor 15 (TST15) assay which is a targeted cancer gene panel dedicated to detecting variants by covering the implicated coding regions of 15 genes associated with solid tumors. TST15 uses next-generation sequencing (NGS) technology to provide a comprehensive assessment of the most commonly mutated genes in 7 cancer types (Lung, Melanoma, Breast, Colon, Ovarian, Gastric, and Prostate). It detects low-frequency variants down to a 5% minor allele frequency (MAF) and requires only 20 ng of DNA isolated from FFPE tumor tissue. TST15 allows for parallel sequencing of multiple cancer-related genes without the need to perform several sequential tests.
FFPE solid tumor tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.
Storage and Transport
Use cold pack during transport of paraffin blocks and unstained slides. Cold pack should not be placed in direct contact with the specimen during shipping.
Specimen Rejection Criteria
All specimens must be submitted according to the test directory instructions. Before submitting specimen, please carefully review specimen requirements.
Listed below are possible causes for delays in specimen processing or possible specimen rejections or cancellations:
- No patient identification on requisition form
- No patient identification on specimen container or slides
- No referring provider
- No account information provided
- No test ordered on requisition
- No specimen source provided
- Inappropriate specimen type
- Insufficient volume for analysis
- Inappropriate specimen container
- Improper specimen transport
- Specimen leaked in transport
- Specimen submitted in incorrect or expired transport media
- Slides broken beyond repair on receipt
- Conflict between patient name on specimen and requisition form
- Specimen not properly preserved
- Sample containing a needle
- FFPE specimens of 5-µm thickness containing tumor content less than 10%
A representative from Client Services will attempt to contact your office to resolve specimen problems. Additional documentation may be requested from your office to be returned by fax. In the event that resolution is not possible, the specimen may be returned to your office.
Turn Around Time (TAT)