Immunofluorescence
Next Generation Sequencing (NGS) · Dermatology
Useful in the diagnosis of suspected autoimmune disease, connective
tissue disease and vasculitis, the direct technique utilizes specific
antibodies that have been conjugated with a fluorochrome (FITC).
The antisera are incubated with the tissue sections, washed and cover
slipped. Using a fluorescent microscope, specific positive staining can
be observed as apple-green staining of the antigenic sites.
Proteins tested: IgG, IgA, IgM, C3, Fibrinogen
tissue disease and vasculitis, the direct technique utilizes specific
antibodies that have been conjugated with a fluorochrome (FITC).
The antisera are incubated with the tissue sections, washed and cover
slipped. Using a fluorescent microscope, specific positive staining can
be observed as apple-green staining of the antigenic sites.
Proteins tested: IgG, IgA, IgM, C3, Fibrinogen
Table of Contents
Assay Description
Useful in the diagnosis of suspected autoimmune disease, connective tissue disease and vasculitis, the direct technique utilizes specific antibodies that have been conjugated with a fluorochrome (FITC). The antisera are incubated with the tissue sections, washed and cover
slipped. Using a fluorescent microscope, specific positive staining can be observed as apple-green staining of the antigenic sites.
Proteins tested: IgG, IgA, IgM, C3, Fibrinogen
Specimen Requirements
Michel's media. If Michel’s fixative is not available, the specimen can be sent overnight in sterile saline (not over a weekend).
Storage and Transport
Refrigerate or room temperature in Michel's fixative.
Specimen Rejection Criteria
All specimens must be submitted according to the test directory instructions. Before submitting specimen, please carefully review specimen requirements.
Listed below are possible causes for delays in specimen processing or possible specimen rejections or cancellations:
- No patient identification on requisition form
- No patient identification on specimen container or slides
- No referring provider
- No account information provided
- No test ordered on requisition
- No specimen source provided
- Inappropriate specimen type
- Insufficient volume for analysis
- Inappropriate specimen container
- Improper specimen transport
- Specimen leaked in transport
- Specimen submitted in incorrect or expired transport media
- Slides broken beyond repair on receipt
- Conflict between patient name on specimen and requisition form
- Specimen not properly preserved
- Sample containing a needle
- FFPE specimens of 5-µm thickness containing tumor content less than 10%
A representative from Client Services will attempt to contact your office to resolve specimen problems. Additional documentation may be requested from your office to be returned by fax. In the event that resolution is not possible, the specimen may be returned to your office.
Turn Around Time (TAT)
3-5 Days.
CPT Codes
88346 (x5)