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Assays

B-Cell Gene Rearrangement

Molecular · Dermatology
Detection of clonal IgH and Ig kappa gene rearragements by PCR of IgH framework regions 1, 2, 3 and joining regions, Ig kappa FR3 and Jk regions.

Table of Contents

Assay Description


Detection of clonal IgH and Ig kappa gene rearragements by PCR of IgH framework regions 1, 2, 3 and joining regions, Ig kappa FR3 and Jk regions.

Specimen Requirements


•Peripheral blood: 4 mL in EDTA tube.

•Bone marrow: 2 mL in EDTA tube.

•FFPE tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.

•Fresh tissue: Two pieces minimum, 0.2 cm3 in RPMI.

Storage and Transport


Refrigerate fresh tissue until shipping. For all specimens, use cold pack for transport. Make sure cold pack is not in direct contact with specimen.

Specimen Rejection Criteria


All specimens must be submitted according to the test directory instructions.  Before submitting specimen, please carefully review specimen requirements.


Listed below are possible causes for delays in specimen processing or possible specimen rejections or cancellations:


  • No patient identification on requisition form
  • No patient identification on specimen container or slides
  • No referring provider
  • No account information provided
  • No test ordered on requisition
  • No specimen source provided
  • Inappropriate specimen type
  • Insufficient volume for analysis
  • Inappropriate specimen container
  • Improper specimen transport
  • Specimen leaked in transport
  • Specimen submitted in incorrect or expired transport media
  • Slides broken beyond repair on receipt
  • Conflict between patient name on specimen and requisition form
  • Specimen not properly preserved
  • Sample containing a needle

A representative from Client Services will attempt to contact your office  to resolve specimen problems.  Additional documentation may be requested from your office to be returned by fax.  In the event that resolution is not possible, the specimen may be returned to your office.

Turn Around Time (TAT)


7 Days.

CPT Codes*


81479(x2)