AFB
Special stain · All
Acid-Fast Bacillus (AFB) Stain Kit is intended to identify acid-fast bacteria, such as Mycobacterium , in tissue sections on the Artisan™ Link and Artisan™ Link Pro Staining Systems. Application of Carbol Fuchsin stains acid-fast bacteria red, followed by decolorization of all tissue elements except the acid-fast bacteria. A Methylene Blue counterstain is then applied to impart a blue color to all background tissue elements.
Table of Contents
Assay Description
Acid-Fast Bacillus (AFB) Stain Kit is intended to identify acid-fast bacteria, such as Mycobacterium , in tissue sections on the Artisan™ Link and Artisan™ Link Pro Staining Systems. Application of Carbol Fuchsin stains acid-fast bacteria red, followed by decolorization of all tissue elements except the acid-fast bacteria. A Methylene Blue counterstain is then applied to impart a blue color to all background tissue elements.
Specimen Requirements
A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type or one (1) unbaked, unstained slide cut at 4-5 microns for H&E staining (required) and two to three (2-3) positively charged unstained slides cut at 3-4 microns for each test/antibody ordered.
Storage and Transport
Use cold pack during transport of paraffin blocks and unstained slides. Cold pack should not be placed in direct contact with the specimen during shipping.
Specimen Rejection Criteria
All specimens must be submitted according to the test directory instructions. Before submitting specimen, please carefully review specimen requirements.
Listed below are possible causes for delays in specimen processing or possible specimen rejections or cancellations:
- No patient identification on requisition form
- No patient identification on specimen container or slides
- No referring provider
- No account information provided
- No test ordered on requisition
- No specimen source provided
- Inappropriate specimen type
- Insufficient volume for analysis
- Inappropriate specimen container
- Improper specimen transport
- Specimen leaked in transport
- Specimen submitted in incorrect or expired transport media
- Slides broken beyond repair on receipt
- Conflict between patient name on specimen and requisition form
- Specimen not properly preserved
- Sample containing a needle
A representative from Client Services will attempt to contact your office to resolve specimen problems. Additional documentation may be requested from your office to be returned by fax. In the event that resolution is not possible, the specimen may be returned to your office.
Turn Around Time (TAT)
2-3 Days.
CPT Codes*
88312